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All Journal HAYATI Journal of Biosciences Jurnal Kedokteran Brawijaya PROSIDING SEMINAR KIMIA UNEJ e-Proceeding BERITA BIOLOGI Jurnal Kimia Riset Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Jurnal Abdi: Media Pengabdian Kepada Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati
AFAF BAKTIR
Chemistry Department, Faculty Of Sciences And Technology Airlangga University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : PROSIDING SEMINAR KIMIA

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THE POTENCY OF PROTEIN EXTRACTS FROMCandida albicans AS BIORECEPTOR ON IMMUNOSENSOR FOR DIAGNOSIS OF CANDIDIASIS Masfufatun -; Noer Kumala; Afaf Baktir
PROSIDING SEMINAR KIMIA PROCEEDING OF INTERNATIONAL CONFERENCE 2015
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to detect C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans through enzimatis and mechanic methods and (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and specific activity 1.96 unit/mg. The snail enzyme hydrolyzed the cell wall of C. albicans with and without sonication, produced planktonic extracell protein extract (PEP) and biofilm extracell proteinextract (BEP), planktonic intracell protein extract (PIP), and biofilm intracell protein extract (BIP), with protein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. The biofilm intracell protein (BIP) showed antigenic property toward antibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguish negative control serum (health man) and positive Candidiasis control by using antigen 1 g/l and 50 l serum.
Co-Authors Akhmaloka Akhmaloka Ali Rohman Ami Soewandi J.S. Andre Pratama Aning Purwaningsih Arju Rohmah Sayyidah Badi’atul Azizah Budi Putri Ayu Andina Budi Putri Ayu Andina, Budi Putri Ayu Dwi Putri Mashfufatur Rohmah Fatiha Khairunnisa Fatiha Khairunnisa Hamami Harini Nur Hikmah Hartati Hartati Hera Lisna Ginawati I GUST MADE SANJAYA KUNTAMAN KUNTAMAN Kuntaman Kuntaman Loo Hariyanto Raharjo, Loo Hariyanto Masfufatun Masfufatun Mei Shirli Yasinta Mochamad Zakki Fahmi Muji Harsini Ni Nyoman Tri Puspaningsih Nira Ambar Arum Noer Kumala Noer Kumala NOER KUMALA Noor Cholies Zaini Nur Cholifah NUR CHOLIFAH Purkan Purkan Putu Oky Ari Tania Siti Wafiroh Sofijan Hadi Sri Sumarsih Sri Sumarsih Sri Sumarsih Syahrani, Achmad Titik Taufikurohmah UNTUNG MURDIYATMO Untung Murdiyatmo Y. Sriwulan M. Zumrotul Koiriyah